Part:BBa_K1022121:Design

pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  Illegal NheI site found at 2317
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 521
  Illegal BglII site found at 2441
  Illegal BamHI site found at 65
  Illegal BamHI site found at 1405
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2626
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119

Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft]

Source

Clonned